STN8 / Anti-Serine/threonine-protein kinase STN8, chloroplastic antibody

  Description:

Rabbit polyclonal antibody

  Background:

STN8 is specific in phosphorylation of N-terminal threonine residues in D1, D2 and CP43 proteins, and Thr-4 in PsbH protein of photosystem II.

  Synonyms:

STN8, STATE TRANSITION 8

  Immunogen:

KLH-conjugated synthetic peptide of (19 aa from C terminal section) derived from Arabidopsis thaliana STN8 (AT5G01920).

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01M sterile PBS.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected / apparent MW: 

55 / 45 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is
100% homologues with the sequence in Medicago truncatula, Brassica napus, Glycine max,
Gossypium raimondii, Brassica rapa, and 80-99% homologues with the sequence in Zea mays,
Panicum virgatum, Triticum aestivum, Hordeum vulgare, Oryza sativa Japonica Group,
Sorghum bicolor, Setaria viridis, Physcomitrium patens, Vitis vinifera, Spinacia oleracea,
Cucumis sativus, Solanum tuberosum, Solanum lycopersicum, Nicotiana tabacum.
For more species homologues information, please contact tech support at [email protected].

Chl: 7.5 μg total chloroplast protein from Arabidopsis thaliana.
TP: 15 μg total protein from Arabidopsis thaliana.
Electrophoresis: 12% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.