PetA / Anti-Cytochrome f antibody

  Description:

Rabbit polyclonal antibody

  Background:

PetA

  Synonyms:

PetA

  Immunogen:

KLH-conjugated synthetic peptide (18 aa from N terminal section) derived from  Synechocystis sp. PCC 6803 PetA (sll1317).

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at [email protected].

  Reconstitution:  

Reconstitution with 150µl of sterile water.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected / apparent MW: 

35 kDa

  Confirmed Reactivity: 

Synechocystis sp. PCC 6803

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Medicago truncatula, Solanum tuberosum, Zea mays, Glycine max, Oryza sativa Japonica Group, Arabidopsis thaliana, Leymus chinensis, Populus trichocarpa, Gossypium raimondii, Hordeum vulgare subsp. spontaneum, Cucumis sativus, Brassica rapa, Brassica napus var. napus, Nicotiana tabacum, Panicum virgatum, Spinacia oleracea, Vitis vinifera, Triticum aestivum, Solanum lycopersicum.
For homologues with other species especially algae, please contact tech support at [email protected].

PCC 6803+L/G: 30 μg whole-cell lysate protein from WT of Synechocystis sp. PCC 6803 (Light Plus Glucose).
PCC6803+G: 30 μg whole-cell lysate protein from WT of Synechocystis sp. PCC 6803 (Dark Plus Glucose).
Electrophoresis: (5-20)% Gradient gel
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.