HPT / Anti-HPT antibody

  Description:

Mouse monoclonal antibody

  Background:

The hygromycin phosphotransferase (denoted hpt, hph or aphIV) gene was originally derived from Escherichia coli. The gene codes for hygromycin phosphotransferase (HPT), which detoxifies the aminocyclitol antibiotic hygromycin B. A large number of plants have been transformed with the hpt gene and hygromycin B has proved very effective in the selection of a wide range of plants, including monocotyledonous. Most plants exhibit higher sensitivity to hygromycin B than to kanamycin, for instance cereals.

  Synonyms:

HPT

  Immunogen:

Synthetic peptide of HPT protein.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150µl of 0.01 M sterile PBS.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

38 kDa

  Confirmed Reactivity: 

  Predicted Reactivity: 

Anti-HPT recognizes transgenic rice.