GPX2 / Anti-Hydroperoxy fatty acid reductase Gpx2 antibody

  Description:

Rabbit polyclonal antibody

  Background:

GPX2 

  Synonyms:

GPX2 

  Immunogen:

KLH-conjugated synthetic peptide (16 aa from C terminal section) derived from  Synechocystis sp. PCC 6803 GPX2 (slr1992).

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at [email protected].

  Reconstitution:  

Reconstitution with 150µl of sterile water.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected / apparent MW: 

17 / 23 kDa

  Confirmed Reactivity: 

Synechocystis sp. PCC 6803

  Predicted Reactivity: 

For homologues with other species especially algae, please contact tech support at [email protected].

PCC 6803+L: 30 μg whole-cell lysate protein from WT of Synechocystis sp. PCC 6803 (Light without Glucose).
PCC 6803+L/G: 30 μg whole-cell lysate protein from WT of Synechocystis sp. PCC 6803 (Light Plus Glucose).
PCC6803+G: 30 μg whole-cell lysate protein from WT of Synechocystis sp. PCC 6803 (Dark Plus Glucose).
Electrophoresis: (5-20)% Gradient gel
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.