TET8 / Anti-Tetraspanin-8 antibody

  Description:

Rabbit polyclonal antibody

  Background:

Tetraspanins are a distinct class of conserved integral proteins. In the Arabidopsis, 17 tetraspanin proteins are identified. They are expressed in different organs/tissues and cell types during embryonic and vegetative development. And the onset of expression coincided with the onset of patterning and cell specification in globular and heart stage embryos (TET1 (AT5G46700), TET3 (AT3G45600), TET4 (AT5G60220), TET5 (AT4G23410), TET8 (AT2G23810), TET10 (AT1G63260), TET13 (AT2G03840), TET14 (AT2G01960), and TET15 (AT5G57810)) and in seedlings at the initiation of the stomatal cell lineage (TET2 (AT2G19580)) or at the asymmetric division in the primary root pericycle upon lateral root initiation (TET13).

  Synonyms:

TET8, TETRASPANIN8

  Immunogen:

KLH-conjugated synthetic peptide of TET8 derived from Arabidopsis thaliana AT2G23810.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at [email protected]

  Reconstitution:  

Reconstitution with 150µl of sterile water.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Applications: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

31 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Brassica napus.
For more species homologues information, please contact tech support at [email protected]

Lane 1: 25 μg total protein from Arabidopsis thaliana leaf.
Lane 2: 50 μg total protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.